Phosphotyrosine antibody

Catalog No. ABIN6938185

The Mouse Monoclonal anti-Phosphotyrosine antibody (Clone PY265) (ABIN6938185) specifically detects Phosphotyrosine in WB, IF and FACS.

Quick Overview for Phosphotyrosine antibody (ABIN6938185)

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PY265

Product Details anti-Phosphotyrosine Antibody

Purification: Purified by Protein A/G

Immunogen: Phosphotyrosine conjugated to BSA

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: MCF-7, MDA-231, T47-D cells. Breast carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Alternative Name: Phosphotyrosine (P-Tyr)

Target Type: Amino Acid

Background: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.