Recombinant ADRP antibody (AA 249-376)

Catalog No. ABIN6938196

The Rabbit Monoclonal anti-ADRP antibody has been validated for WB, ELISA, Coat and IHC (f). It is suitable to detect ADRP in samples from Human.

Quick Overview for Recombinant ADRP antibody (AA 249-376) (ABIN6938196)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ADRP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ADFP-2755R

Product Details anti-ADRP Antibody

Binding Specificity: AA 249-376

Specificity: Recognizes a protein of 48 kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA),Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Alternative Name: PLIN2

Background: Adipophilin, ADFP, Adipose differentiation-related protein (ADRP), Perilipin-2 (PLIN2),Adipophilin / Perilipin-2 (Marker of Lipid Accumulation)
Cellular localisation: Cytoplasmic

Molecular Weight: 48kDa

Gene ID: 123, 3416

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism