POMC antibody (AA 162-176)

Catalog No. ABIN6938219

This Mouse Monoclonal antibody specifically detects POMC in IHC (f). It exhibits reactivity toward Human.

Quick Overview for POMC antibody (AA 162-176) (ABIN6938219)

Target: POMC (Proopiomelanocortin (POMC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POMC antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: CLIP-1407

Product Details anti-POMC Antibody

Binding Specificity: AA 162-176

Specificity: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to CLIP (aa25-39 of ACTH), does not react with Synacthen (aa1-24 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to (aa 162-176) of human ACTH (NGAEDESAEAFPLEF)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Normal pituitary gland or pituitary tumor

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for POMC

Target: POMC (Proopiomelanocortin (POMC))

Alternative Name: POMC

Background: Adrenocorticotropin, alpha, beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin, beta Endorphin, beta or gamma Lipotropin (LPH), CLIP, Met Enkephalin, POC, POMC,ACTH (Adrenocorticotrophic Hormone) (Pituitary Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: ACTH is ~5kDa, and the POMC precursor is ~30kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

Gene ID: 5443, 1897

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour