Recombinant PTH antibody (AA 32-115)

Catalog No. ABIN6938263

This anti-PTH antibody is a Rabbit Monoclonal antibody detecting PTH in IHC (f). Suitable for Human.

Quick Overview for Recombinant PTH antibody (AA 32-115) (ABIN6938263)

Target: PTH (Parathyroid Hormone (PTH))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PTH antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: PTH-2295R

Product Details anti-PTH Antibody

Binding Specificity: AA 32-115

Specificity: Epitope of this MAb maps in between aa 1-34. PTH is a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion.It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment around aa 32-115 of human mature-PTH-polypeptide (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human parathyroid gland carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PTH

Target: PTH (Parathyroid Hormone (PTH))

Alternative Name: PTH

Target Type: Hormone

Background: HPTH, Parathormone, Parathyrin, Parathyroid hormone 1 (PTH1), Parathyroid hormone (PTH),Parathyroid Hormone (PTH) (N-Terminal)
Cellular localisation: Cytoplasmic and Secreted

Molecular Weight: 9kDa

Gene ID: 5741, 37045

UniProt: P01270

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process