PTH antibody (AA 1-115)

Catalog No. ABIN6938265

The Rabbit Polyclonal anti-PTH antibody has been validated for IHC (f). It is suitable to detect PTH in samples from Human.

Quick Overview for PTH antibody (AA 1-115) (ABIN6938265)

Target: PTH (Parathyroid Hormone (PTH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTH antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-PTH Antibody

Binding Specificity: AA 1-115

Specificity: Epitope of this PAb maps in the N-terminus of PTH, a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion.It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.

Cross-Reactivity (Details): Human. Predicted to react with Mouse. Rat. Rabbit. Cow. Dog. Pig. Deer. Orangutan.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment around aa 1-115 of human mature-PTH-polypeptide (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human parathyroid gland carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PTH

Target: PTH (Parathyroid Hormone (PTH))

Alternative Name: PTH

Target Type: Hormone

Background: HPTH, Parathormone, Parathyrin, Parathyroid hormone 1 (PTH1), Parathyroid hormone (PTH),Parathyroid Hormone (PTH) (N-Terminal)
Cellular localisation: Cytoplasmic and Secreted

Molecular Weight: 9kDa

Gene ID: 5741, 37045

UniProt: P01270

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process