s100a4 antibody (AA 1-200)

Catalog No. ABIN6938334

The Mouse Monoclonal anti-s100a4 antibody has been validated for WB, IHC, IF, FACS and IHC (f). It is suitable to detect s100a4 in samples from Human.

Quick Overview for s100a4 antibody (AA 1-200) (ABIN6938334)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: S100A4-1481

Product Details anti-s100a4 Antibody

Binding Specificity: AA 1-200

Specificity: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Cross-Reactivity (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PANC-1, HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4

Background: S100A4, S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein,S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)
Cellular localisation: Cytoplasmic and nuclear

Molecular Weight: 10-12kDa

Gene ID: 6275, 654444

UniProt: P26447

Pathways: S100 Proteins