s100a4 antibody

Catalog No. ABIN6938337

The Mouse Monoclonal anti-s100a4 antibody has been validated for WB, IF and FACS. It is suitable to detect s100a4 in samples from Human.

Quick Overview for s100a4 antibody (ABIN6938337)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CPTC-S100A4-3

Product Details anti-s100a4 Antibody

Specificity: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100A4 has been implicated in the progression and prognosis of several forms of human cancer, e.g. breast, colorectal, gastric, pancreatic and bladder cancer, SCLC and oesophageal squamous cell carcinoma, among others. Poor prognosis associated with high S100A4 expression is accompanied by clear signs of disease progression, e.g. high histological and clinical grades and involvement of lymph nodes.Also indicative of poor prognosis is high S100A4 expression coupled with reduced E-cadherin expression in pancreatic, oral squamous cell carcinoma and in melanoma. S100A4 expression is inversely related with expression of metastasis suppressor nm23 and with prognosis of breast cancer. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human S100A4 full length protein

Isotype: IgG2c kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: T98G, HeLa, A549 or A375 cells. Placenta, small intestine.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4

Background: S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein,S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)
Cellular localisation: Nucleus and cytoplasm.

Molecular Weight: 12kDa

Gene ID: 6275, 654444

UniProt: P26447

Pathways: S100 Proteins