Band 3/AE1 antibody

Catalog No. ABIN6938401

The Mouse Monoclonal anti-Band 3/AE1 antibody (Clone Q1-156) (ABIN6938401) specifically detects Band 3/AE1 in WB, ELISA, IF and Coat. The antibody is reactive with Human samples.

Quick Overview for Band 3/AE1 antibody (ABIN6938401)

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Band 3/AE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat)

Clone: Q1-156

Product Details anti-Band 3/AE1 Antibody

Specificity: Band 3, also designated AE1, is an erythrocyte membrane glycoprotein that contributes to cell stuctural integrity and mediates exchange of chloride and bicarbonate across the phospholipid bilayer. The diverse functions of the approximately 900 amino acid protein are mediated by two distinct domains. The amino-terminal domain, also known as cdb3 for cytoplasmic domain of erthrocyte membrane band 3, acts as an attachment site for the erythrocyte skeleton by binding ankyrin. The carboxy-terminal, membrane-associated domain carries out exchange transport of anions. Degradation of band 3 can generate an aging antigen known as senescent cell antigen, or SCA, which is expressed on old cells and marks them for removal by the immune system. An isoform of band 3, which lacks the first 65 amino acids and does not bind ankryin, is expressed in kidney.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human fetal liver cells.

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Red blood cell lysate, Human colon and lung tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Band 3/AE1

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Alternative Name: SLC4A1

Background: AE1, Anion exchange protein 1, BND3, CD233, DI, Diego blood group, EMPB3, EPB3, Erythrocyte membrane protein band 3, Erythroid anion exchange protein, FR, Froese blood group, RTA1A, SLC4A1, Solute carrier family 4 member 1, SW, Swann blood group, Waldner blood group, WD1, WR, Wright blood group antibody,Band III
Cellular localisation: Cell membrane

Molecular Weight: 95kDa

Gene ID: 6521, 210751, 443948

UniProt: P02730