Recombinant SPTA1 antibody (AA 356-475)

Catalog No. ABIN6938439

The Rabbit Monoclonal anti-SPTA1 antibody has been validated for WB, ELISA, Coat and IHC (f). It is suitable to detect SPTA1 in samples from Human.

Quick Overview for Recombinant SPTA1 antibody (AA 356-475) (ABIN6938439)

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SPTA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SPTA1-2939R

Product Details anti-SPTA1 Antibody

Binding Specificity: AA 356-475

Specificity: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human SPTA (around aa356-475) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K562, HeLa, Jurkat cells. Placenta or Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SPTA1

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Alternative Name: SPTA1

Background: Alpha I spectrin, Elliptocytosis 2 (EL2), Erythrocyte alpha spectrin, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha erythrocytic 1, SPH3, SPTA1,Spectrin Alpha 1 (Erythrocyte Marker)
Cellular localisation: Cytoplasmic and Cell Surface

Molecular Weight: 240kDa

Gene ID: 6708, 119825

UniProt: P02549

Pathways: Regulation of Actin Filament Polymerization