Spectrin beta Chain, Erythrocyte (SPTB) antibody

Catalog No. ABIN6938442

The Mouse Monoclonal anti- antibody has been validated for WB, IF, IHC, ELISA, Coat and IHC (f). It is suitable to detect in samples from Human.

Quick Overview for Spectrin beta Chain, Erythrocyte (SPTB) antibody (ABIN6938442)

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RG-26

Product Details

Specificity: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Red cell membranes

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Alternative Name: SPTB

Background: Beta-I spectrin, EL3, erythrocyte, HS2, SPTB1, HSPTB1, membrane cytoskeletal protein, spectrin beta (erythrocytic),Spectrin beta I (SPTB)
Cellular localisation: Cell Surface

Molecular Weight: 246kDa

Gene ID: 6710, 26915

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization