Recombinant EpCAM antibody (Extracellular Domain)

Catalog No. ABIN6938496

The Rabbit Monoclonal anti-EpCAM antibody is suitable to detect EpCAM in samples from Human. It has been validated for FACS and IHC (f).

Quick Overview for Recombinant EpCAM antibody (Extracellular Domain) (ABIN6938496)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: EGP40-2571R

Product Details anti-EpCAM Antibody

Binding Specificity: AA 100-224, Extracellular Domain

Specificity: Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Cross-Reactivity (Details): Human. Does not react with Dog or Cat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells). Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HT29 cells. Breast tumor.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: TACSTD1

Background: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1),Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker)
Cellular localisation: Cell surface

Molecular Weight: 40-43kDa

Gene ID: 4072, 542050

UniProt: P16422