Transferrin Receptor antibody

Catalog No. ABIN6938535

The Mouse Monoclonal anti-Transferrin Receptor antibody has been validated for FACS, ELISA, IF and Coat. It is suitable to detect Transferrin Receptor in samples from Human.

Quick Overview for Transferrin Receptor antibody (ABIN6938535)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Coating (Coat)

Clone: 66IG10

Product Details anti-Transferrin Receptor Antibody

Specificity: It recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Its epitope is localized in the extracellular domain of CD71. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human thymocytes (T-cells)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: CCRF-CEM, Jurkat or K-562. Human placenta or breast carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: TFRC

Background: Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR,CD71 / Transferrin Receptor (TFRC)
Cellular localisation: Cell surface

Molecular Weight: 85-95kDa (monomer), 190kDa (dimer)

Gene ID: 7037, 529618

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis