Thyroglobulin antibody

Catalog No. ABIN6938560

This anti-Thyroglobulin antibody is a Mouse Monoclonal antibody detecting Thyroglobulin in IHC, FACS and StM. Suitable for Human, Mouse and Rat.

Quick Overview for Thyroglobulin antibody (ABIN6938560)

Target: Thyroglobulin (TG)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: 2H11

Product Details anti-Thyroglobulin Antibody

Specificity: MAb 2H11 reacts with a partially defined epitope of human thyroglobulin. This epitope is different form the epitope recognized by MAb 6E1. Thyroglobulin is a 660 kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by and processed within the thyroid gland to produce the hormone thyroxine and triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary and follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid and lung neoplasms.

Purification: Purified by Protein A/G

Immunogen: Human thyroid follicular cells

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Thyroid.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL, 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Alternative Name: Thyroglobulin (Thyroidal Cell Marker)

Molecular Weight: 660kDa (Dimeric Form)

Gene ID: 7038

UniProt: P01266

Pathways: Thyroid Hormone Synthesis