TIMP2 antibody (AA 48-220)

Catalog No. ABIN6938578

This anti-TIMP2 antibody is a Mouse Monoclonal antibody detecting TIMP2 in ELISA, IHC (f) and Coat. Suitable for Human.

Quick Overview for TIMP2 antibody (AA 48-220) (ABIN6938578)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Clone: TIMP2-2044

Product Details anti-TIMP2 Antibody

Binding Specificity: AA 48-220

Specificity: It recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human TIMP2 protein fragment (aa 48-220) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2

Background: Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, Tissue Inhibitor of Metalloproteinase 2,TIMP2 (Tissue Inhibitor of Metalloproteinase 2)
Cellular localisation: Cytoplasm and Cell Surface

Molecular Weight: 21kDa

Gene ID: 7077, 633514

UniProt: P16035

Pathways: cAMP Metabolic Process