CD137 antibody

Catalog No. ABIN6938626

The Rat Monoclonal anti-CD137 antibody (Clone LOB12) (ABIN6938626) specifically detects CD137 in FACS, IF and Func. The antibody is reactive with Mouse samples.

Quick Overview for CD137 antibody (ABIN6938626)

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD137 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone: LOB12

Product Details anti-CD137 Antibody

No Cross-Reactivity: Human

Purification: Purified by Protein A/G

Immunogen: Murine CD137 human Fc fusion protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: MPBMC's or Thymus.

Known Application: Functional Studies (Order Antibody without BSA & Azide); Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: TNFRSF9

Background: CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival. The LOB12.3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling and delay tumor growthin vivowhen administered in combination with immune checkpoint inhibitors.

Molecular Weight: 39kDa

Gene ID: 21942

UniProt: P20334

Pathways: Cancer Immune Checkpoints