p63 antibody (AA 5-17)

Catalog No. ABIN6938658

The Rabbit Polyclonal anti-p63 antibody has been validated for WB and IHC (f). It is suitable to detect p63 in samples from Human.

Quick Overview for p63 antibody (AA 5-17) (ABIN6938658)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p63 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-p63 Antibody

Binding Specificity: AA 5-17

Specificity: P40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Cross-Reactivity (Details): Human. Mouse. Rat. Cow.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

Isotype: IgG

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: HEK293 cells. Prostate Carcinoma or Lung Squamous Cell Carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Alternative Name: TP63

Background: DeltaNp63, TP63,p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker)
Cellular localisation: Nuclear

Molecular Weight: 40kDa

Gene ID: 8626, 137569

UniProt: Q9H3D4