p63 antibody

Catalog No. ABIN6938660

This Mouse Monoclonal antibody specifically detects p63 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for p63 antibody (ABIN6938660)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p63 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TP63-11

Product Details anti-p63 Antibody

Specificity: P63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands, however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90 % . Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80 %. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.

Cross-Reactivity (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human p63 protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HEK293 cells. Prostate Carcinoma or Lung or bladder squamous cell carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Alternative Name: TP63

Background: Amplified in squamous cell carcinoma (AIS), Chronic ulcerative stomatitis protein (CUSP), EEC3, Keratinocyte transcription factor KET, LMS, NBP, p40, P51/P63, p53 like transcription factor, p53-related protein p63, RHS, SHFM4, TAp63alpha, TP53CP, TP53L, TP63, TP73, TP73L, Transformation-related protein 63, Trp53rp1, Trp6,3, Tumor protein 63, Tumor protein p53-like, tumor protein p73-like,p63 (Squamous, Basal & Myoepithelial Cell Marker)
Cellular localisation: Nuclear

Molecular Weight: 63kDa

Gene ID: 8626, 137569

UniProt: Q9H3D4