TPSAB1 antibody (AA 115-233)

Catalog No. ABIN6938670

The Mouse Monoclonal anti-TPSAB1 antibody has been validated for IHC (f). It is suitable to detect TPSAB1 in samples from Human.

Quick Overview for TPSAB1 antibody (AA 115-233) (ABIN6938670)

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TPSAB1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TPSAB1-1961

Product Details anti-TPSAB1 Antibody

Binding Specificity: AA 115-233

Specificity: Tryptases comprise a family of trypsin-like serine proteases (peptidase family S1). Tryptases are stored in mast cell secretory granules and basophils. Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Tryptases are released into the extracellular environment and are resistant to all known endogenous proteinase inhibitors. This antibody reacts with mast cells distributed in skin, synovium, lung, and heart. This antibody does not bind with any other cell type. Human mast cell tryptase is considered to be an important marker of mast cell activation and is an important mediator of inflammation. Mastocytosis is a term collectively used for a group of disorders in which there is abnormal accumulation of mast cells in one or multiple organs. Anti-tryptase, combined with anti-CD2, anti-CD25, and anti-CD117, can be useful in identifying reactive mast cell hyperplasia, myelogenous neoplasms, mast cell leukemia, and mastocytosis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Tryptase protein fragment (around aa 115-233) (Exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Skin or Tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TPSAB1

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Alternative Name: TPSAB1

Background: Lung Tryptase, Mast cell alpha II Tryptase, Mast cell beta I Tryptase, Mast cell protease 7, Mast cell protease II, MCP7, Pituitary Tryptase, Skin Tryptase, TPS1, TPSAB1, TPSB1, Tryptase 1, Tryptase alpha 1, Tryptase skin,Tryptase (Mast Cell Marker)
Cellular localisation: Secreted and Cytoplasmic

Molecular Weight: 31-36kDa

Gene ID: 7177, 405479

UniProt: Q15661