Transferrin antibody (AA 311-445)

Catalog No. ABIN6938674

This anti-Transferrin antibody is a Mouse Monoclonal antibody detecting Transferrin in ELISA, IHC, Coat and StM. Suitable for Human.

Quick Overview for Transferrin antibody (AA 311-445) (ABIN6938674)

Target: Transferrin (TF)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Clone: TF-3001

Product Details anti-Transferrin Antibody

Binding Specificity: AA 311-445

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 311-445) of human TF protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Jurkat, HeLa, MCF-7 or K562 cells. Human liver.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS without BSA and without Azide.

Preservative: Azide free

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: Transferrin (Early Marker of Oligodendrocytes)

Background: Iron (Fe) is a tightly metabolically controlled mineral and growth factor present in all living cells. Iron not bound in erythrocyte hemoglobin is transported by transferrin (Tf), the iron transport protein of vertebrate serum. The transferrin protein contains two homologous domains, each of which contain an Fe-binding site. The majority of transferrin is synthesized in the liver and secreted into the blood, but it is also produced in lower amounts in testis and brain as well as in oligodendrocytes, where transferrin is an early marker of oligodendrocyte differentiation. From the blood, transferrin is internalized by erythroblasts and reticulocytes upon binding the transferrin receptor (TfR), also designated CD71, through a system of coated pits and vesicles. After Fe release, transferrin is returned to the extracellular medium, where it can be reused. Defects in the transferrin gene results in atransferrinemia, a rare autosomal recessive disorder characterized by microcytic anemia and iron loading.

Molecular Weight: 79kDa

Gene ID: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis