Thymidine Phosphorylase antibody

Catalog No. ABIN6938686

This anti-Thymidine Phosphorylase antibody is a Mouse Monoclonal antibody detecting Thymidine Phosphorylase in WB, IP and IHC (f). Suitable for Human.

Quick Overview for Thymidine Phosphorylase antibody (ABIN6938686)

Target: Thymidine Phosphorylase (TYMP)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thymidine Phosphorylase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: P-GF-44C

Product Details anti-Thymidine Phosphorylase Antibody

Specificity: Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Cross-Reactivity (Details): Human. Mouse. Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunoprecipitation (0.5-1 μg/500 μg protein lysate), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HUVEC cells. Breast,Spleen, Bladder, Lung or Kaposi tumors.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Alternative Name: TYMP

Background: ECGF, ECGF1, Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PD-ECGF, PDECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine phosphorylase, TP, Tymp,Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker)
Cellular localisation: Cytoplasmic and Nuclear

Molecular Weight: 55kDa

Gene ID: 1890, 180903

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2