Ubiquitin B antibody (AA 1-119)

Catalog No. ABIN6938717

This Mouse Monoclonal antibody specifically detects Ubiquitin B in WB, IF, FACS and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Ubiquitin B antibody (AA 1-119) (ABIN6938717)

Target: Ubiquitin B (UBB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: UBB-1748

Product Details anti-Ubiquitin B Antibody

Binding Specificity: AA 1-119

Specificity: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)

Isotype: IgG2c kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HeLa or Jurkat cells. Alzheimer's Brain.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Ubiquitin B

Target: Ubiquitin B (UBB)

Alternative Name: UBB

Background: Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2,Ubiquitin (Autophagy Marker)
Cellular localisation: Cell Surface, Cytoplasmic and Nuclear

Molecular Weight: 9kDa

Gene ID: 7314, 356190, 520348

UniProt: P62979, P62987, P62988

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway