VISTA antibody

Catalog No. ABIN6938764

The Mouse Monoclonal anti-VISTA antibody (Clone VISTA-2865) (ABIN6938764) specifically detects VISTA in ELISA, IHC, Coat and IHC (f). The antibody is reactive with Human samples.

Quick Overview for VISTA antibody (ABIN6938764)

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VISTA antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: VISTA-2865

Product Details anti-VISTA Antibody

Specificity: VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human VISTA protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human spleen or placenta tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for VISTA

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Alternative Name: VSIR

Background: B7H5, DD1alpha, GI24, PD-1H, PDCD1 homolog, Platelet receptor Gi24, PP2135, Stress-induced secreted protein 1 (SISP1), V domain Ig suppressor of T cell activation, V Set Immuno-regulatory Receptor,VISTA / GI24 (Negative Regulator of Immune Response)
Cellular localisation: Membrane and cytoplasm.

Molecular Weight: 34kDa

Gene ID: 64115, 47382

UniProt: Q9H7M9

Pathways: Cancer Immune Checkpoints