WT1 antibody (AA 1-181)

Catalog No. ABIN6938782

This anti-WT1 antibody is a Mouse Monoclonal antibody detecting WT1 in IHC (f). Suitable for Human.

Quick Overview for WT1 antibody (AA 1-181) (ABIN6938782)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 6F-H2

Product Details anti-WT1 Antibody

Binding Specificity: AA 1-181

Specificity: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Cross-Reactivity (Details): Human. Rat. Weakly reacts with Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant protein corresponding to residues 1-181 of human WT1.

Isotype: IgG1 kappa

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: K562 cells. Kidney, Testis, Wilm's Tumor or Mesothelioma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background: WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1,Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)
Cellular localisation: Nuclear and Cytoplasmic

Molecular Weight: 47-55kDa

Gene ID: 7490, 591980

UniProt: P19544

Pathways: Tube Formation