TUBB3 antibody (AA 437-450)

Catalog No. ABIN6939054

This anti-TUBB3 antibody is a Mouse Monoclonal antibody detecting TUBB3 in WB and IHC (f). Suitable for Human and Synthetic.

Quick Overview for TUBB3 antibody (AA 437-450) (ABIN6939054)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Human, Synthetic

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TUBB3-3731

Product Details anti-TUBB3 Antibody

Binding Specificity: AA 437-450

Specificity: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Cross-Reactivity (Details): Human. Others not known.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide (aa 437-450) of human Tubulin beta 3 protein (TUBB3), coupled to KLH.

Isotype: IgG1

Application Details

Application Notes:

Positive Control: U87, A375, Raji, HEP2 cells. Brain and Testis

Known Application: Western Blotting (1-2 μg/mL),Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: TUBB3

Background: Beta 3 tubulin, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, M(beta)3, M(beta)6, MC1R, Neuron specific beta III Tubulin, Neuron-specific class III beta-tubulin, TUBB3, TUBB4, Tubulin beta-3, Tubulin beta-4, Tubulin beta-III,Tubulin beta 3 / TUBB3 (Neuronal & Stem Cell Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 55kDa

Gene ID: 10381, 511743

UniProt: Q13509

Pathways: Microtubule Dynamics, M Phase