CDX2 antibody (AA 150-249)

Catalog No. ABIN6939057

The Mouse Monoclonal anti-CDX2 antibody has been validated for ELISA, Coat, IHC (f) and ISt. It is suitable to detect CDX2 in samples from Human.

Quick Overview for CDX2 antibody (AA 150-249) (ABIN6939057)

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDX2 antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Clone: CDX2-1690

Product Details anti-CDX2 Antibody

Binding Specificity: AA 150-249

Specificity: The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoidsand is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma.However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

Cross-Reactivity (Details): Human,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa150-249) of human CDX2 protein (exact sequence is proprietary)

Isotype: IgG2a

Application Details

Application Notes:

Positive Control: HT29 cells. Colon Carcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for CDX2

Target: CDX2 (Caudal Type Homeobox 2 (CDX2))

Alternative Name: CDX2

Background: Caudal type homeobox 2, Caudal type homeobox transcription factor 2, Caudal-type homeobox protein 2, CDX2,CDX2 / Caudal Type Homeobox 2 (GI Epithelial Marker)
Cellular localisation: Nuclear

Molecular Weight: 40kDa

Gene ID: 1045, 174249

UniProt: Q99626

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance