Chromogranin A antibody

Catalog No. ABIN6939126

This anti-Chromogranin A antibody is a Mouse Monoclonal antibody detecting Chromogranin A in IHC and StM. Suitable for Human, Rat, Mouse, Monkey and Pig.

Quick Overview for Chromogranin A antibody (ABIN6939126)

Target: Chromogranin A (CHGA)

Reactivity: Human, Rat, Mouse, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: LK2H10

Product Details anti-Chromogranin A Antibody

Specificity: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

No Cross-Reactivity: Guinea Pig, Rabbit, Sheep (Ovine)

Purification: Purified by Protein A/G

Immunogen: Human pheochromocytoma

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: PC12 cells. Adrenal gland, bowel, thyroid, pancreas or pheochromocytoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: CHGA

Molecular Weight: 68-75kDa

Gene ID: 1113

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling