CNN1 antibody

Catalog No. ABIN6939167

The Rabbit Polyclonal anti-CNN1 antibody has been validated for FACS, IF, IHC and StM. It is suitable to detect CNN1 in samples from Human and Rat.

Quick Overview for CNN1 antibody (ABIN6939167)

Target: CNN1 (Calponin 1 (CNN1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNN1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Product Details anti-CNN1 Antibody

Specificity: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this antibody reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Purification: Purified by Protein A/G

Immunogen: Recombinant human CNN1 protein

Isotype: IgG

Application Details

Application Notes:

Positive Control: Myoepithelial cells in breast ducts.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CNN1

Target: CNN1 (Calponin 1 (CNN1))

Alternative Name: CNN1

Molecular Weight: 34kDa

Gene ID: 1264

UniProt: P51911