CTNND1 antibody

Catalog No. ABIN6939213

This anti-CTNND1 antibody is a Mouse Monoclonal antibody detecting CTNND1 in ELISA, IF, FACS and Coat. Suitable for Human, Mouse and Rat.

Quick Overview for CTNND1 antibody (ABIN6939213)

Target: CTNND1 (Catenin (Cadherin-Associated Protein), delta 1 (CTNND1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNND1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Clone: 25a

Product Details anti-CTNND1 Antibody

Purification: Purified by Protein A/G

Immunogen: Mouse p120 Catenin (pY96)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells. HeLa whole cell lysate.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CTNND1

Target: CTNND1 (Catenin (Cadherin-Associated Protein), delta 1 (CTNND1))

Alternative Name: CTNND1

Background: The membrane associated protein pp120 Src substrate (p120 Catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation. It shares structural similarity with the Drosophila Armadillo protein and the vertebrate beta-catenin and gamma-catenin proteins. Its characteristic Arm domain that is composed of 42-amino acid motif repeats evidences this similarity. In the cell, p120 Catenin is localized to the E-Cadherin/catenins cell adhesion complex. Like beta- and gamma-catenin, p120 Catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain and may similarly interact with other Cadherins. It exists as four isoforms that range in size from 90-115 kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions. Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 Catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation. Thus, p120 Catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signaling molecules, such as PTP1C.

Molecular Weight: 120kDa

Gene ID: 1500

UniProt: O60716

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling