Decorin antibody (AA 212-336)

Catalog No. ABIN6939230

This anti-Decorin antibody is a Mouse Monoclonal antibody detecting Decorin in IHC and StM. Suitable for Human.

Quick Overview for Decorin antibody (AA 212-336) (ABIN6939230)

Target: Decorin (DCN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: DCN-3523

Product Details anti-Decorin Antibody

Binding Specificity: AA 212-336

Purification: Purified by Protein A/G

Immunogen: Recombinant human Decorin protein fragment (aa212-336) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Human prostate or skin tissue (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: DCN

Background: Decorin is a small leucine-rich proteoglycan (SLRP) family member that consists of a glycosaminoglycan chain-containing core protein. The core protein contains ten leucine rich repeats that contain sites for glycosylation, flanked by disulfide bond stabilizing loops. Decorin binds to Collagen Type I, II and IV in vivo and promotes the formation of fibers with variations in stability and solubility. The Decorin core protein binds to growth factors, intercellular matrix molecules, such as Fibronectin and Thrombospondin, and to the Decorin endocytosis receptor. Decorin binds to and inhibits TGF and is a direct or indirect negative modulator of TGF synthesis. Inhibition of Decorin core protein gene expression by the combination of IFN- and TNF may contribute to cartilage destruction that is characteristic of inflammatory joint diseases. The human Decorin gene maps to chromosome 12q21.33 and encodes a 359 amino acid protein.

Molecular Weight: 43kDa

Gene ID: 1634

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process