Desmocollin 2 antibody (Extracellular Domain)

Catalog No. ABIN6939250

The Mouse Monoclonal anti-Desmocollin 2 antibody has been validated for WB, IHC, IF, FACS and StM. It is suitable to detect Desmocollin 2 in samples from Human, Mouse and Rat.

Quick Overview for Desmocollin 2 antibody (Extracellular Domain) (ABIN6939250)

Target: Desmocollin 2 (DSC2)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmocollin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: 7G6

Product Details anti-Desmocollin 2 Antibody

Binding Specificity: Extracellular Domain

Purification: Purified by Protein A/G

Immunogen: Human desmocollin-2 extracellular domain

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Skin tissue.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Desmocollin 2

Target: Desmocollin 2 (DSC2)

Alternative Name: DSC2

Background: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers.

Molecular Weight: 110kDa (DSC2), 100kDa (DSC3)

Gene ID: 1824

UniProt: Q02487