ALDH1A1 antibody (AA 315-434)

Catalog No. ABIN6939366

This Mouse Monoclonal antibody specifically detects ALDH1A1 in WB, IHC and StM. It exhibits reactivity toward Human.

Quick Overview for ALDH1A1 antibody (AA 315-434) (ABIN6939366)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALDH1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: ALDH1A1-1381

Product Details anti-ALDH1A1 Antibody

Binding Specificity: AA 315-434

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 315-434) of human ALDH1A1 protein (exact sequence is proprietary).

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Liver, K562, Lung, HepG2 or A549 cells. Colon or Testicular Carcinoma.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1

Background: ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.

Molecular Weight: 56kDa

Gene ID: 216

UniProt: P00352

Pathways: Dopaminergic Neurogenesis