FAT1 antibody (Cytoplasmic Domain)

Catalog No. ABIN6939370

This anti-FAT1 antibody is a Mouse Monoclonal antibody detecting FAT1 in ELISA, WB, Coat and IF. Suitable for Drosophila melanogaster.

Quick Overview for FAT1 antibody (Cytoplasmic Domain) (ABIN6939370)

Target: FAT1 (FAT Tumor Suppressor Homolog 1 (FAT1))

Reactivity: Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAT1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Coating (Coat), Immunofluorescence (IF)

Clone: FAT1-3D7-1

Product Details anti-FAT1 Antibody

Binding Specificity: Cytoplasmic Domain

Cross-Reactivity (Details): Expected to cross-react with Chimpanzee. Rat (92 % . 85 % identity with immunogen. respectively) due to sequence homology.

Purification: Purified by Protein A/G

Immunogen: Cytoplasmic domain of Drosophila Fat protein.

Isotype: IgM kappa

Application Details

Application Notes:

Positive Control: Wild type imaginal discs from third instar Drosophila larvae.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FAT1

Target: FAT1 (FAT Tumor Suppressor Homolog 1 (FAT1))

Alternative Name: FAT1

Background: The FAT proteins are members of the Cadherin superfamily homologous to the Drosophila Fat protein that functions as a positive regulator of planar cell polarity in the Drosophila wing. FAT1 is an unusual cadherin that controls cell growth and planar polarity while acting as a tumor suppressor. FAT1 is a proximal element of a signaling pathway that determines both cellular polarity in the plane of the monolayer and directed actin-dependent cell motility. FAT1 is localized at the leading edge of lamellipodia, filopodia and microspike tips where it directly interacts with Ena/VASP proteins to regulate the actin polymerization complex. When targeted to mitochondrial outer leaflets, the cytoplasmic domain of FAT1 recruits components of the actin polymerization machinery sufficient to induce ectopic actin polymerization. FAT1 expression in vascular smooth muscle cells (VSMCs) increases significantly after arterial injury or growth factor stimulation, implicating FAT1 in the control of VSMC functions central to vascular remodeling by facilitating migration and limiting proliferation. FAT1 is also involved in psychic disorders, and its action may be of patho-physiological importance.

Molecular Weight: 500kDa

Gene ID: 2195

UniProt: Q14517