Recombinant Filaggrin antibody (AA 198-288)

Catalog No. ABIN6939387

The Mouse Monoclonal anti-Filaggrin antibody has been validated for IHC, ISt and StM. It is suitable to detect Filaggrin in samples from Human.

Quick Overview for Recombinant Filaggrin antibody (AA 198-288) (ABIN6939387)

Target: Filaggrin (FLG)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Filaggrin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone: RFLG-1561

Product Details anti-Filaggrin Antibody

Binding Specificity: AA 198-288

Purification: Purified by Protein A/G

Immunogen: Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: U-251-MG cells. Skin.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Filaggrin

Target: Filaggrin (FLG)

Alternative Name: FLG

Background: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Molecular Weight: 26-45kDa (Processed), 350kDa (Profilaggrin)

Gene ID: 2312

UniProt: P20930

Pathways: Sensory Perception of Sound, Stem Cell Maintenance