MLANA antibody

Catalog No. ABIN6939391

The Mouse Monoclonal anti-MLANA antibody has been validated for IF, FACS, IHC, ISt and StM. It is suitable to detect MLANA in samples from Human and Horse.

Quick Overview for MLANA antibody (ABIN6939391)

Target: MLANA (Melan A (MLANA))

Reactivity: Human, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone: SPM342

Product Details anti-MLANA Antibody

Specificity: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Purified by Protein A/G

Immunogen: Recombinant hMART-1 protein

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: MLANA

Molecular Weight: 20-22kDa (doublet)

Gene ID: 2315

UniProt: Q16655