Frataxin antibody (AA 57-210)

Catalog No. ABIN6939442

The Mouse Monoclonal anti-Frataxin antibody has been validated for WB, IHC and StM. It is suitable to detect Frataxin in samples from Human.

Quick Overview for Frataxin antibody (AA 57-210) (ABIN6939442)

Target: Frataxin (FXN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Frataxin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: FXN-2124

Product Details anti-Frataxin Antibody

Binding Specificity: AA 57-210

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human Frataxin (FXN) protein (around aa 57-210) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: HepG2, Panc-2, HL-60 or HEK293 cells. Pancreas and Liver.

Known Application: Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Frataxin

Target: Frataxin (FXN)

Alternative Name: FXN

Background: Frataxin is a nuclear-encoded mitochondrial protein implicated in Friedreich's ataxia (FRDA), a human autosomal recessive neurodegenerative disease. Lack of Frataxin causes iron to accumulate in the mitochondrial matrix suggesting that Frataxin is involved in mitochondrial iron homeostasis and possibly in iron transport. Frataxin has an alpha-beta fold consisting of two helices flanking an antiparallel beta sheet.

Molecular Weight: 14kDa

Gene ID: 2395

UniProt: Q16595

Pathways: Transition Metal Ion Homeostasis, Regulation of Cell Size