ALPL antibody

Catalog No. ABIN6939448

This Mouse Monoclonal antibody specifically detects ALPL in IHC and StM. It exhibits reactivity toward Human and Cow.

Quick Overview for ALPL antibody (ABIN6939448)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This ALPL antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: ALPL-597

Product Details anti-ALPL Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant human ALPL protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Intestine.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: ALPL

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.

Molecular Weight: 55kDa

Gene ID: 249

UniProt: P05186