PLAP antibody

Catalog No. ABIN6939455

This anti-PLAP antibody is a Mouse Monoclonal antibody detecting PLAP in IHC and ISt. Suitable for Human.

Quick Overview for PLAP antibody (ABIN6939455)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAP antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunostaining (ISt)

Clone: GM022

Product Details anti-PLAP Antibody

Specificity: Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Purification: Purified by Protein A/G

Immunogen: Recombinant human PLAP protein

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: HepG2 cells. Placenta or seminoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: ALPP

Molecular Weight: 70kDa

Gene ID: 250

UniProt: P05187