Recombinant FTL antibody (AA 38-165)

Catalog No. ABIN6939463

This Rabbit Monoclonal antibody specifically detects FTL in WB, IHC and StM. It exhibits reactivity toward Human.

Quick Overview for Recombinant FTL antibody (AA 38-165) (ABIN6939463)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FTL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: FTL-2338R

Product Details anti-FTL Antibody

Binding Specificity: AA 38-165

Purification: Purified by Protein A/G

Immunogen: Recombinant human FTL protein fragment (around aa 38-165) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Positive Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Known Application: Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: FTL

Background: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Molecular Weight: 19-25kDa

Gene ID: 2512

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis