GAD antibody (AA 72-135)

Catalog No. ABIN6939482

The Mouse Monoclonal anti-GAD antibody has been validated for WB, ELISA, FACS and Coat. It is suitable to detect GAD in samples from Human.

Quick Overview for GAD antibody (AA 72-135) (ABIN6939482)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Coating (Coat)

Clone: GAD1-2563

Product Details anti-GAD Antibody

Binding Specificity: AA 72-135

Specificity: This MAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD's are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

Purification: Purified by Protein A/G

Immunogen: Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: T98G or K-562 or HEK293 cells. Pancreas.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Western Blot (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1

Molecular Weight: 67kDa

Gene ID: 2571

UniProt: Q99259