GJB1 antibody

Catalog No. ABIN6939515

The Mouse Monoclonal anti-GJB1 antibody is suitable to detect GJB1 in samples from Human, Rat and Mouse. It has been validated for WB, FACS, IF and IHC (fro).

Quick Overview for GJB1 antibody (ABIN6939515)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M12-13

Product Details anti-GJB1 Antibody

Specificity: This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.

Immunogen: Rat junctional complexes

Isotype: IgG

Application Details

Application Notes:

Positive Control: MCF-7 cells. Liver, Kidney, Stomach or Tonsil.

Known Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Frozen) (0.5-1.0 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Alternative Name: GJB1

Molecular Weight: 27-32kDa

Gene ID: 2705

UniProt: P08034

Pathways: Cell-Cell Junction Organization