IL3RA antibody (AA 26-171)

Catalog No. ABIN6939783

This Mouse Monoclonal antibody specifically detects IL3RA in IHC, ISt and StM. It exhibits reactivity toward Human.

Quick Overview for IL3RA antibody (AA 26-171) (ABIN6939783)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL3RA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone: IL3RA-1531

Product Details anti-IL3RA Antibody

Binding Specificity: AA 26-171

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human IL3RA protein (around aa 26-171) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: THP-1, RPMI-8226 or HDLM-2 cells. Tonsil, Lymph Node or Stomach.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: IL3RA

Background: CD123 is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudo-autosomal region on chromosomes X or Y.

Molecular Weight: ~70kDa

Gene ID: 3563

UniProt: P26951

Pathways: JAK-STAT Signaling