PLK1.S antibody

Catalog No. ABIN6939848

This Mouse Monoclonal antibody specifically detects PLK1.S in Coat, ELISA and WB. It exhibits reactivity toward Xenopus laevis.

Quick Overview for PLK1.S antibody (ABIN6939848)

Target: PLK1.S (Serine/threonine-Protein Kinase PLK1 (PLK1.S))

Reactivity: Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1.S antibody is un-conjugated

Application: Coating (Coat), ELISA, Western Blotting (WB)

Clone: AZ44

Product Details anti-PLK1.S Antibody

No Cross-Reactivity: Human, Mouse (Murine)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length PLK1 Xenopus laevis protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Mitotic CSF Xenopus egg extract.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PLK1.S

Target: PLK1.S (Serine/threonine-Protein Kinase PLK1 (PLK1.S))

Alternative Name: plk1.S

Background: Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and Cdc5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle, Plk protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Useful for confirming that extracts have maintained CSF status and have not yet been activated into interphase.

Molecular Weight: 66kDa

Gene ID: 380481

UniProt: P70032