KRT15 antibody

Catalog No. ABIN6939914

The Mouse Monoclonal anti-KRT15 antibody is suitable to detect KRT15 in samples from Human. It has been validated for WB, IHC and FACS.

Quick Overview for KRT15 antibody (ABIN6939914)

Target: KRT15 (Keratin 15 (KRT15))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: KRT15-2958

Product Details anti-KRT15 Antibody

Purpose: Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker) Antibody

Specificity: Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human KRT15 protein

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: A431 cells. Skin, Thymus, Colon or Prostate.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for KRT15

Target: KRT15 (Keratin 15 (KRT15))

Alternative Name: KRT15

Background:

CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15

A431 cells. Skin, Colon or Prostate., Thymus

Molecular Weight: 52kDa

Gene ID: 3866, 654570

UniProt: P19012