LHB antibody

Catalog No. ABIN6939960

The Mouse Monoclonal anti-LHB antibody has been validated for IHC and StM. It is suitable to detect LHB in samples from Human.

Quick Overview for LHB antibody (ABIN6939960)

Target: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LHB antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: LHb-1214

Product Details anti-LHB Antibody

Purification: Purified by Protein A/G

Immunogen: Recombinant beta sub-unit of human LH

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Anterior Pituitary.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL, 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for LHB

Target: LHB (Luteinizing Hormone beta Polypeptide (LHB))

Alternative Name: LHB

Target Type: Hormone

Background: Luteinizing hormone (LH) is a glycoprotein. Each monomeric unit is a sugar-like protein molecule, two of these make the full, functional protein. Its structure is similar to the other glycoproteins, follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits that are connected by two bridges. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. LH has a beta subunit of 121 amino acids (LHB) that confers its specific biologic action and is responsible for interaction with the LH receptor. This beta subunit contains the same amino acids in sequence as the beta subunit of hCG and both stimulate the same receptor, however, the hCG beta subunit contains an additional 24 amino acids and the hormones differ in the composition of their sugar moieties.LH is synthesized and secreted by gonadotrophs in the anterior lobe of the pituitary gland. In concert with the other pituitary gonadotropin follicle-stimulating hormone (FSH), it is necessary for proper reproductive function. In the female, an acute rise of LH levels triggers ovulation. In the male, where LH has also been called Interstitial Cell-Stimulating Hormone (ICSH), it stimulates Leydig cell production of testosterone. LH is a useful marker in classification of pituitary tumors and the study of pituitary disease.

Molecular Weight: 22kDa

Gene ID: 3972

UniProt: P01229

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process