LMO2 antibody (AA 23-140)

Catalog No. ABIN6939966

This Mouse Monoclonal antibody specifically detects LMO2 in WB, IHC, FACS and StM. It exhibits reactivity toward Human.

Quick Overview for LMO2 antibody (AA 23-140) (ABIN6939966)

Target: LMO2 (LIM Domain Only 2 (Rhombotin-Like 1) (LMO2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LMO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Clone: LMO2-1971

Product Details anti-LMO2 Antibody

Binding Specificity: AA 23-140

Purification: Purified by Protein A/G

Immunogen: Recombinant human LMO2 protein fragment (around aa 23-140) (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: K562, Ramos or Raji cells. Placenta, pancreas or Hodgkin s lymphoma.

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10mM PBS with 0.05% BSA & 0.05% azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for LMO2

Target: LMO2 (LIM Domain Only 2 (Rhombotin-Like 1) (LMO2))

Alternative Name: LMO2

Background: The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. It has a particular function in normal and lymphatic endothelial cells involving the regulation of angiogenesis and lymph-angiogenesis. Immunohistochemical studies have also demonstrated expression of LMO2 in both normal germinal center B-cells and germinal center-derived B-cell lymphomas, including follicular lymphoma and diffuse large B-cell lymphoma.The use of anti-LMO2 is valuable as a tool in the identification of lymphomas of B-cell origin. LMO2 is useful in differentiating follicular lymphoma (LMO2+) from nodal marginal zone lymphoma (LM02-). It also is positive in Hodgkin s and Burkitt s lymphomas.

Molecular Weight: 24kDa

Gene ID: 4005

UniProt: P25791

Pathways: Chromatin Binding