LSP1 antibody (AA 174-278)

Catalog No. ABIN6939969

This Mouse Monoclonal antibody specifically detects LSP1 in IHC, IHC (p) and StM. It exhibits reactivity toward Human.

Quick Overview for LSP1 antibody (AA 174-278) (ABIN6939969)

Target: LSP1 (Lymphocyte-Specific Protein 1 (LSP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LSP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Staining Methods (StM)

Clone: LSP1-3025

Product Details anti-LSP1 Antibody

Binding Specificity: AA 174-278

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human LSP1 protein (around aa174-278) (exact sequence is proprietary)

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Human tonsil, colon, spleen or Hodgkin's lymphoma (IHC-P).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for LSP1

Target: LSP1 (Lymphocyte-Specific Protein 1 (LSP1))

Alternative Name: LSP1

Background: LSP1 is a hematopoietic-expressed gene that encodes an F-actin-binding, leukocyte-specific (including B and T lymphocytes, granulocytes and macrophages), phosphoprotein. However, mRNA splice variants that do not encode the lympho-specific protein are expressed from this gene in nonlymphoid cell lines as well (myocytes, stromal cells and fibroblasts), suggesting pp52 has a divergent role in signal transduction. The pp52 (LSP1) locus maps to human chromosome 11p15.5, which is implicated in tumor-related chromosomal translocations found in chronic lymphocytic leukemia. The pp52 promoter contains key elements that control transcriptional activity including an initiator specifying the unique 5' terminus of pp52 mRNA, tandem pairs of Ets and SP1 motifs, and a single C/EBP motif. LSP1 binds the cytoskeleton and has been implicated in affecting cytoskeletal remodeling in a variety of leukocyte functions, including cell motility and chemotaxis.

Molecular Weight: 52kDa

Gene ID: 4046

UniProt: P33241