Moesin antibody

Catalog No. ABIN6940082

This anti-Moesin antibody is a Mouse Monoclonal antibody detecting Moesin in WB, IHC, IF, FACS and StM. Suitable for Human and Rat.

Quick Overview for Moesin antibody (ABIN6940082)

Target: Moesin (MSN)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Moesin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: MSN-491

Product Details anti-Moesin Antibody

Specificity: Recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human Moesin protein

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: K562, HT-29, CH3LC or HUVEC cells. Uterus, Placenta, Tonsil, Melanoma, Testicular Carcinoma.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: MSN

Molecular Weight: 78kDa

Gene ID: 4478

UniProt: P26038

Pathways: Asymmetric Protein Localization