Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

NEFH antibody

This Mouse Monoclonal antibody specifically detects NEFH in WB, FACS, ELISA, IHC (fro), StM and Coat. It exhibits reactivity toward Human, Rat, Mouse, Chicken and Pig.
Catalog No. ABIN6940181

Quick Overview for NEFH antibody (ABIN6940181)

Target

See all NEFH Antibodies
NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity

  • 117
  • 86
  • 65
  • 49
  • 30
  • 23
  • 14
  • 12
  • 9
  • 9
  • 9
  • 7
  • 7
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Human, Rat, Mouse, Chicken, Pig

Host

  • 70
  • 63
  • 9
Mouse

Clonality

  • 88
  • 54
Monoclonal

Conjugate

  • 93
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NEFH antibody is un-conjugated

Application

  • 90
  • 51
  • 37
  • 30
  • 27
  • 23
  • 21
  • 18
  • 16
  • 14
  • 13
  • 13
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Staining Methods (StM), Coating (Coat)

Clone

SPM563
  • Specificity

    This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

    Purification

    Purified by Protein A/G

    Immunogen

    Human recombinant neurofilament protein

    Isotype

    IgG1 kappa
  • Application Notes

    Positive Control: Brain, Neuroblastoma.

    Known Application: ELISA (For coating, order Ab without BSA), Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Frozen & formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Expiry Date

    24 months
  • Target

    NEFH (Neurofilament, Heavy Polypeptide (NEFH))

    Alternative Name

    NEFH

    Molecular Weight

    200kDa

    Gene ID

    4744

    UniProt

    P12036
You are here:
Chat with us!