PAX2A antibody (AA 223-354)

Catalog No. ABIN6940252

This anti-PAX2A antibody is a Mouse Monoclonal antibody detecting PAX2A in ELISA, IHC, Coat and StM. Suitable for Human.

Quick Overview for PAX2A antibody (AA 223-354) (ABIN6940252)

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX2A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Clone: PAX2-1104

Product Details anti-PAX2A Antibody

Binding Specificity: AA 223-354

Specificity: Recognizes a protein of 42 kDa, which is identified as PAX2. It is a member of the paired box family of transcription factors, which is required for development and proliferation of the kidney, brain, and mullerian organs. PAX2 genes contain a highly conserved DNA sequence within the paired box region, which encodes a DNA-binding domain, enabling PAX proteins to bind the promoters of specific genes to transcriptionally regulate their expression. PAX2 is specifically expressed in the developing central nervous system, eye, ear, and urogenital tract, and is essential for the development of these organs. In normal adult tissues PAX2 was mainly detected in the urogenital system, including kidney, ureteric epithelium, fallopian tube epithelium, ovary and uterus. In tumors, PAX2 has been detected in renal cell carcinomas, Wilms' tumors, nephrogenic adenomas and papillary serous carcinoma of the ovary. PAX2 has been used as a marker for the identification of renal cell carcinoma and ovarian carcinoma by immunohistochemistry.

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 223-354) human PAX2 protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: NAMALWA cells. Fetal kidney or Renal Cell Carcinoma (RCC) or Ovarian Carcinoma.

Known Application: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PAX2A

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Alternative Name: PAX2

Molecular Weight: 42kDa

Gene ID: 5076

UniProt: Q02962

Pathways: Carbohydrate Homeostasis, Stem Cell Maintenance, Tube Formation