Cytochrome C antibody (AA 1-80)

Catalog No. ABIN6940320

The Mouse Monoclonal anti-Cytochrome C antibody has been validated for WB, IHC, FACS, IF and StM. It is suitable to detect Cytochrome C in samples from Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster and Frog.

Quick Overview for Cytochrome C antibody (AA 1-80) (ABIN6940320)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse, Horse, Dog, Pigeon, Drosophila melanogaster, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone: SPM389

Product Details anti-Cytochrome C Antibody

Binding Specificity: AA 1-80

Specificity: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Purification: Purified by Protein A/G

Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: CYCS

Molecular Weight: 15kDa

Gene ID: 54205

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity